Vitamin E provides protection against in vitro oxidative stress

Over the centuries, humans have developed many ingenious methods in their attempts to control herbs, weeds, insects, invertebrates and microorganisms that constantly threatened the supply of food and fibre as well as posed a threat to public health. The aim of present study was to find out the protective nature of antioxidant property of vitamin E in against chlorpyrifos and endosulfan toxicity. Erythrocytes were collected from healthy goat and exposed to 10ppm chlorpyrifos and endosulfan pesticide individually and also along with vitamin E treatment. Results showed endosulfan was more toxic in comparison of chlorpyrifos. Activity of Superoxide dismutase (SOD) and Catalase significantly decreased and lipid peroxidation (LPO) and Glutathione S-transfarase (GST) was increased in comparison of control value. The result of the present study suggests that vitamin E is an effective antioxidant for chlorpyrifos and endosulfan pesticide toxicity in reducing oxidative stress burden. It can be concluded that organophosphate chlorpyrifos and endosufan insecticides induced oxidative stress and lipid peroxidation level in mammalian erythrocytes (RBCs) and conjugation and supplementation of vitamin E or in combination has amelionated these effects.